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American Foundation for
Donation & Transplantation

8154 Forest Hill Ave, Suite 3
Richmond, VA 23235
Phone: (804) 323-9890
Toll Free: (800) 543-6399
FAX: (804) 323-1300


American Foundation for Donation & Transplantation
Molecular Typing 2000 Manual


The first edition of this manual, published in 1994, provided guidance in the strange, new world of HLA typing with DNA, primers, and probes. The field has moved very rapidly since 1994, and now it is time for a new edition which reflects the progress that has been made and gives direction for the future.

The first section of the 2000 edition addresses the concepts that undergird molecular methods. The second section is procedure-oriented but emphasizes the "why" of the methodology rather than the "how to". Remaining sections include recommendations about how to set up a DNA laboratory, quality assurance and quality control issues, suggestions about the way to approach an NMDP donor search, helpful and relevant web sites, and the current ASHI standards.

CONTENTS

INTRODUCTION TO CONCEPTS
  • Basic Molecular Biology
  • Immunobiology of HLA
  • Comparison of Molecular and Serological HLA Typing-Editorial
  • HLA and Transplantation
    • Allogeneic Marrow Transplantation
    • Solid Organ Transplantation
  • Polymerase Chain Reaction as the Basis for Detection of HLA Genes

     

PROCEDURES FOR DNA TYPING
  • Preparation of Material for Molecular Typing
  • Molecular Typing Using Sequence-Specific Primers
  • Laboratory Protocols for In-House PCR-SSP
  • Commercial Products for HLA Typing by PCR-SSP
  • Molecular Typing Using Sequence-Specific Oligonucleotide Probes
  • HLA Class I Molecular Typing by Sequence Specific Oligonucleotide Probes
  • Reverse Dot Blot DNA Typing Technique for HLA-DRB and DQB
  • HLA-DRB Typing Using an In-House ELISA-Based Oligoprobe Assay
  • Sequence-Based Typing
  • Trouble Shooting

LABORATORY DESIGN AND METHOD SELECTION

QUALITY ASSURANCE IN THE DNA LABORATORY

SEARCHING FOR A MARROW DONOR THROUGH THE NMDP: SUGGESTIONS FOR DIRECTING A SEARCH

INTERNET TIPS
  • Helpful Web Sites
  • Downloading Sequence Data from the Internet

ASHI STANDARDS, 1998


To order a copy call AFDT or mail a copy of the
order form and a check to:

8154 Forest Hill Ave, Suite 3
Richmond, VA 23235
Phone: (804) 323-9890
Toll Free: (800) 543-6399
FAX: (804) 323-1300

 




 

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